Hiranmoy Das Laboratory

About Us

Vascular Biology and Stem Cell Research Laboratory

Stem cell therapy using autologous stem cells to treat degenerative diseases has been promising, however the limited availability and compromised quality of progenitor cells in aged and diseased patients limit its therapeutic potential. Alternatively, use of cord blood-derived stem cells is advantageous as it is easy to harvest, harmless to donor, ethical, ontogenetically primitive and can be stored in cord blood bank for years. Moreover, cord blood-derived stem cell transplantation is associated with reduced risk of developing graft versus host disease. Nevertheless, small number of stem cells provided by a single cord limits its clinical application. Our laboratory has developed a nanofiber-based ex-vivo human umbilical cord blood-derived stem cell expansion technology, which not only preserves stem cell phenotype, but also provides required number of biologically functional stem cells. Furthermore, we can genetically modify nanofiber-expanded stem cells to enhance their angiogenic and therapeutic potential for various degenerative diseases such as hind limb ischemia, myocardial ischemia, stroke-mediated ischemia, wound healing and osteoporosis. Current investigations use immunocompromised murine, rat and swine models for relevant studies. Molecular aspects of stem cell functionality are also being investigated in these animal models after cell-based therapy. We are also extending our in-depth research on human dental pulp-derived stem cells, corneal epithelial and endothelial stem cells to make them suitable for clinical use for various degenerative diseases.

The molecular mechanisms regulating activation and functionality of monocytes remain incompletely understood in the context of inflammation. We provided evidence that a transcription factor, Kr眉ppel-like factor 2 (KLF2), inhibits proinflammatory activation of monocytes. KLF2 expression in circulating monocytes is reduced in patients with chronic inflammatory conditions such as coronary artery disease (CAD). KLF2 inhibits the LPS-mediated induction of proinflammatory factors, cytokines, and chemokines and reduces phagocytosis in vitro. Conversely, short interfering RNA-mediated reduction in KLF2 increased inflammatory gene expression. Reconstitution of immunodeficient mice with KLF2-overexpressing monocytes significantly reduced carrageenan-induced acute paw edema formation. Mechanistically, we show that KLF2 inhibits the transcriptional activity of both NF-kB and activator protein 1, in part by means of recruitment of transcriptional coactivator p300/CBP-associated factor. These observations identify KLF2 as a novel negative regulator of monocytic activation. Current investigations focus on the role of KLF2 in the genetically altered murine model of rheumatoid arthritis.

Human gamma delta (纬未) T cells represent a small subset of T cell population that possesses distinct T cell receptor (TCR) on their surface. Majority of 纬未 T cells are V纬2V未2 subset. This subset can increase 2- to 10-fold in peripheral blood in a variety of infectious diseases. V纬2V未2 T cells may be considered part of the adaptive immune system as they have a memory phenotype, junctionally diverse TCR鈥檚 that require gene rearrangement for their cell surface expression and the ability to undergo either anergy or expansion depending on the availability of co-stimulation. On the other hand, V纬2V未2 T cells are also considered a part of the innate immune response. Pattern recognition by the V纬2V未2 TCR allows the expansion of memory 纬未 T cells into a large number in normal adults during microbial infections. These large numbers of memory T cells are capable of responding to antigens produced by microbes and thus may bridge the gap between the innate and adaptive immune responses. MHC class I chain related molecules A and B (MICA and MICB, danger signals), which are widely expressed in epithelial tumor cells, and virally or bacterially infected cells, can be recognized by 纬未 T cells and NK cells via NKG2D; a signaling pathway responsible for enhanced cytotoxicity against infected or tumor cells. Our current focus is to identify molecules responsible for recognition of various tumor cells (such as ovarian, breast, and cervical) by 纬未 T cells. The ultimate goal is to develop combined targeted immunotherapy with 纬未 T cells for various tumors.

                        
                        Research Interest
                        
                        KLF2
Stem cells
Myeloid cells
Autophagy
Gamma delta T cells

                        

The Team

Derek Barthels

Ph.D. Candidate/ Research Assistant

Md Sariful Islam Howlader
Graduate Student/ Research Assistant

Prathyusha Naidu

Graduate Student / Research Assistant

Honors & Awards

2022

Recipient of President鈥檚 Excellence in Research Award from the Texas Tech University Health Sciences Center, TX, USA.
Tenured Professor, Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX.
Received P3 Teaching team of the year award, Integrated Therapy and Practice VII; Oncology (PharmD Course), Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX.
Recipient of Chancellor鈥檚 Council Distinguished Research Award from the Texas Tech University Health Sciences Center, the highest honor granted to faculty throughout the Texas Tech University System, TX, USA.

2021

Elected Fellow of American Heart Association (FAHA) conferred by the Council on Basic Cardiovascular Sciences (BCVS), USA.

2020

Inducted into the Arthritis, Connective Tissue and Skin (ACTS) Study Section, NIH, as a 鈥楥hartered Member鈥�.

2018

Inducted into the National Academy of Inventors, USA.
Keynote Speaker, Second International Conference on Clinical Trial and Innovative Therapeutics. Organized by The South African Training Academy held at Durban, South Africa.

Adarsh Nambiar, BS student, ABRI Summer Program Student, 2022.
Prateeksha FNU, Postdoctoral Fellow, 2021- Present.
Jaganmay Sarkar, Postdoctoral Fellow, 2021- 2022.
William Chen, BS student, ABRI Summer Program Student, 2021.
Prathyusha Naidu, PhD student, Graduate School of Biomedical Sciences, 香蕉直播, 2020-Present.
Md. Sariful Islam Howlader, PhD student, Graduate School of Biomedical Sciences, 香蕉直播, 2020-Present.
Ashmita Pramanik, PhD, Postdoctoral Fellow, 2020- 2021.
Ripon Sarkar, PhD, Postdoctoral Fellow, 2019- 2021.
Sneham Tiwari, PhD, Postdoctoral Fellow, 2019- 2020.
Sudhir Kshirsagar, PhD, Postdoctoral Fellow, 2018- 2020.
Jyotirindra Maity, PhD, Postdoctoral Fellow, 2018- 2020.
Taru Bharadwaj, BS student, ABRI Summer Program Student, 2018.
Suman Kanji, PhD, Postdoctoral Fellow, 2018- 2019.
Sarah Anderson, PhD student, Graduate School of Biomedical Sciences, 香蕉直播, 2017-Present.
Derek Barthels, PhD student, Graduate School of Biomedical Sciences, 香蕉直播, 2017- Present.
Dipranjan Laha, PhD, Postdoctoral Fellow, 2017- 2019.
Moonmoon Deb, PhD, Postdoctoral Fellow, 2017- 2018.
Prerana Jha, PhD, Postdoctoral Fellow, 2017- 2018.
Daniela Rolph, PhD student, Graduate School of Biomedical Sciences, 香蕉直播, 2016- 2021.
Carl Greene, PhD student, Graduate School of Biomedical Sciences, 香蕉直播, 2016- Present.
Arijit Bhowmik, PhD, Postdoctoral Fellow, 2016- 2017.
Prachi Bansal, Second year medical student, NEOMED, 2016.

NIH (NIAMS) - 04/01/2016 鈥� 03/31/2023.
Myeloid KLF2 regulation mechanisms in rheumatoid arthritis
$ 1.694 Million
Role: PI
NIH (NEI) - 09/01/2020 鈥� 08/31/2024.
Regenerative Therapy for Corneal Endothelial Dystrophies and Injuries
The goal of this project is to evaluate possible corneal endothelial stem cells for treatment of corneal endothelial dystrophies.
$ 2.3 Million
Role: PI
CPRIT - 2020- 2025.
Whole-animal imaging to super resolution microscopy: An imaging core for the 香蕉直播 campus at Amarillo.
$2,831,213
Role: Co-PI

Prateeksha P, Naidu P, Das M, Barthels D, and Das H. KLF2 regulates neural differentiation of dental pulp-derived stem cells by modulating autophagy and mitophagy. Submitted 2022.
Sarkar J, Das M, Howlader SI, Prateeksha P, Barthels D, and Das H. Epigallocatechin-3-gallate inhibits osteoclastic differentiation by modulating mitophagy and mitochondrial functions. Cell Death and Disease- In Press, 2022. (IF: 9.705)
Barthels D, Prateeksha P, Nozohouri S, Villalba H, Zhang Y, Sharma S, Anderson S, Howlader SI, Nambiar A, Abbruscato TJ, and Das H. Dental pulp-derived stem cells preserve astrocyte health during induced gliosis by modulating mitochondrial activity and functions. Cellular and Molecular Neurobiology. 2022. https://doi.org/10.1007/s10571-022-01291-8. (IF: 4.231)
Laha D搂, Sarkar J搂, Maity J搂, Pramanik A, Howlader SI, Barthels D, and Das H. Polyphenolic compounds inhibit osteoclast differentiation while reducing autophagy through limiting ROS and the mitochondrial membrane potential. Biomolecules, 2022, 12 (9), 1220; https://doi.org/10.3390/biom12091220. (IF: 6.064)
Anderson S, Prateeksha P, and Das H. Dental pulp-derived stem cells reduce inflammation accelerate wound healing, and mediate M2 polarization of myeloid cells. Biomedicines 2022, 10(8): 1999. https://doi.org/10.3390/biomedicines10081999. (IF: 4.757)
Greene CJ搂, Anderson S搂, Barthels D搂, Howlader SI搂, Kanji S, Sarkar J and Das H. DPSC products accelerate wound healing in diabetic mice through induction of SMAD molecules. Cells, 2022, 11(15): 2409. https://doi.org/10.3390/cells11152409. (IF: 7.666)
Maity J, Barthels D, Sarkar J, Prateeksha, P, Deb M, Rolph D, and Das H. Ferutinin induces osteoblast differentiation of DPSCs via induction of KLF2 and autophagy/mitophagy. Cell Death and Disease. 2022 May 12;13(5):452. doi: 10.1038/s41419-022-04903-9. (IF: 9.705)
Ashraf-Uz-Zaman M, Ji G, Tidwell D, Yin L, Thakolwiboon S, Pan J, Shahi S, Barthels D, Sajib SM, Trippier PC, Mikelis CM, Das H, Avila M, Neugebauer V, German NA. Evaluation of urea-based inhibitors of the dopamine transporter using the experimental autoimmune encephalomyelitis model of multiple sclerosis. ACS Chemical Neuroscience. 2022 Jan 19;13(2):217-228. doi: 10.1021/acschemneuro.1c00647. Epub 2022 Jan 3. PMID: 34978174. (IF: 5.780)
Rolph D, and Das H. K/BxN serum-induced arthritis and assessment of osteoclastogenesis. 鈥淢ethods in Molecular Biology: Osteoclast Biology. Springer Nature. Submitted. 2021.
Rolph D, and Das H. In vitro evaluation of murine bone marrow-derived osteoclast activity. 鈥淢ethods in Molecular Biology: Osteoclast Biology. Springer Nature. Submitted. 2021.
Deb M, Laha D, Maity J, and Das H. SETD2-mediated epigenetic regulation of noncanonical Wnt5A during osteoclastogenesis. Clinical Epigenetics. 2021 Oct 18;13(1):192. doi: 10.1186/s13148-021-01125-2.PMID: 34663428. (IF: 7.291)
Rush SW, Chain JL, and Das H. Corneal epithelial stem cell supernatant in the treatment of severe dry eye disease: a pilot study. Clinical Ophthalmology. 16 July 2021 Volume 2021:15 Pages 3097-3107. DOI https://doi.org/10.2147/OPTH.S322079. (IF: 0.74)
Klionsky DJ,鈥�.. Das H鈥︹€t al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition), Autophagy, 2021. DOI: 10.1080/15548627.2020.1797280; https://doi.org/10.1080/15548627.2020.1797280. (IF: 13.391)
Kanji S, Greene CJ, and Das H. Dental pulp-derived stem cells inhibit osteoclast differentiation by secreting osteoprotegerin and deactivating AKT signaling in monocytes. Journal of Cellular and Molecular Medicine. 2021 Jan 28. doi: 10.1111/jcmm.16071. (IF: 5.295)
Anderson S and Das H. Cutaneous wound generation in diabetic NOD/SCID mice and the use of nanofiber-expanded hematopoietic stem cell therapy. Methods in Molecular Biology: Wound Regeneration. 2021;2193:41-48. doi: 10.1007/978-1-0716-0845-6_5. PubMed PMID: 32808257.
Barthels D and Das H. Generation of acute hind limb ischemia in NOD/SCID mice and treatment with nanofiber-expanded CD34+ hematopoietic stem cells. Methods in Molecular Biology: Wound Regeneration. 2021;2193:121-128. doi: 10.1007/978-1-0716-0845-6_12. PubMed PMID: 32808264.
Greene C and Das H. Development of cutaneous wound in diabetic immunocompromised mice and use of dental pulp-derived stem cell product for healing. Methods in Molecular Biology: Wound Regeneration. 2021;2193:23-30. doi: 10.1007/978-1-0716-0845-6_3. PubMed PMID: 32808255.
Rolph D and Das H. Generation of myocardial ischemic wounds and healing with stem cells. Methods in Molecular Biology: Wound Regeneration. 2021;2193:141-147. doi: 10.1007/978-1-0716-0845-6_14. PubMed PMID: 32808266.
Maity J, Deb M, Greene C, and Das H. KLF2 regulates dental pulp-derived stem cell differentiation through the induction of mitophagy and altering mitochondrial metabolism. Redox Biology. 2020 Sep; 36: 101622. doi: 10.1016/j.redox.2020.101622. PMID: 32777717. PMCID: PMC7417940. (IF: 10.787)
Rolph D and Das H. Transcriptional regulation of osteoclastogenesis: the emerging role of KLF2. Frontiers in Immunology 2020. 13 May, Vol 11, article 937. https://doi.org/10.3389/fimmu.2020.00937 (IF: 8.786)
Ranjan A, Ramachandran S, Gupta N, Kaushik I, Wright S, Srivastava S, Das H, Srivastava S, Sahdeo P, and Srivastava SK. Role of phytochemicals in cancer prevention. International Journal of Molecular Sciences. 2019, 20, 4981; doi:10.3390/ijms20204981. (IF: 6.208)
Das M, Deb M, Laha D, Joseph M, Kanji S, Aggarwal R, Iwenofu H, Pompili VJ, Jarjour W, and Das H. Myeloid Kr眉ppel-like factor 2 critically regulates K/BxN serum-induced arthritis. Cells, 2019, 8(8), 908; https://doi.org/10.3390/cells8080908. (IF: 7.666)
Kanji S, Das M, Joseph M, Aggarwal R, Sharma S, Ostrowski M, Pompili VJ, Mao HQ, and Das H. Nanofiber-expanded human CD34+ cells heal cutaneous wounds in streptozotocin-induced diabetic mice. Scientific Reports, 2019, Jun 10;9(1):8415. doi: 10.1038/s41598-019-44932-7. (IF: 4.996)
Laha D, Deb M and Das H. KLF2 (Kr眉ppel-like factor 2 [lung]) regulates osteoclastogenesis by modulating autophagy. Autophagy. 2019; 15(12), 2063-2075. doi: 10.1080/15548627.2019.1596491. (IF: 13.391)
Lustberg M, Reinbolt R, Addison D, Rupert A, Moore S, Carothers S, Suresh A, Das H, Berger M, Ramaswamy B, Wesolowski R, Binkley P, Raman SV, and Shapiro CL. Early Detection of Anthracycline-Induced Cardiotoxicity in Breast Cancer Survivors with T2 Cardiac Magnetic Resonance. Circulation: Cardiovascular Imaging, 2019 May;12(5):e008777. doi: 10.1161/CIRCIMAGING.118.008777. (IF: 8.589)
Barthels D and Das H. Current advances in ischemic stroke research and therapies. BBA-Molecular Basis of Disease. 2018. https://doi.org/10.1016/j.bbadis.2018.09.012. (IF: 6.633)
Das M, Laha D, Kanji S, Joseph M, Aggarwal R, Iwenofu H, Pompili VJ, Jain MK, and Das H. Induction of Kr眉ppel-like factor 2 reduces K/BxN serum-induced arthritis. Journal of Cellular and Molecular Medicine, 23:1386鈥�1395, 2019. http://doi: 10.1111/jcmm.14041. (IF: 5.295)
Rolph DN, Deb M, Greene CJ, Das M, Joseph M, Aggarwal R, Leblebicioglu B and Das H. Ferutinin directs dental pulp-derived stem cells towards the osteogenic lineage by epigenetically regulating canonical Wnt signaling. BBA-Molecular Basis of Disease, 2018. pii: S0925-4439(18)30435-6. https://doi.org/10.1016/j.bbadis.2018.10.032. (IF: 6.633)
Jha P and Das H. KLF2 in regulation of NF-kB-mediated immune cell function and inflammation. International Journal of Molecular Sciences. 2017 Nov 10;18(11). pii: E2383. doi: 10.3390/ijms18112383. (IF: 6.208)
Kanji S and Das H. Advances of stem cell therapeutics in cutaneous wound healing and regeneration. Mediators of Inflammation, 2017; Article ID 5217967, 14 pages, https://doi.org/10.1155/2017/5217967. (IF: 4.529)
Joseph M, Das M, Kanji S, Lu J, Aggarwal R, Chakroborty D, Sarkar C, Yu H, Mao HQ, Basu S, Pompili VJ, and Das H. Retention of stemness and vasculogenic potential of human umbilical cord blood stem cells after repeated expansions on PES-nanofiber matrices. Biomaterials. 2014; 35:8566-8575. doi: 10.1016/j.biomaterials.2014.06.037. Epub 2014 Jul 4. PubMed PMID: 25002260; PubMed Central PMCID: PMC4131920. (IF: 15.304)
Lu J, Das M, Kanji S, Aggarwal R, Joseph M, Ray A, Shapiro CL, Pompili VJ, and Das H. Induction of ATM/ATR pathway combined with V纬2V未2 T cells enhance cytotoxicity of ovarian cancer cells. 2014. BBA-Molecular Basis of Disease. 2014; 1842:1071-1079. doi: 10.1016/j.bbadis.2014.04.003. Epub 2014 Apr 13. (IF: 6.633)
Aggarwal R, Pompili VJ, and Das H. Generation of osteoporosis in immunocompromised mice for stem cell therapy in Animal Models for Stem Cell Therapy: Methods in Molecular Biology. 1213: 209-214, 2014. DOI: 10.1007/978-1-4939-1453-1_17.
Kanji S, Das M, Aggarwal R, Lu J, Joseph M, Pompili VJ and Das H. Nanofiber-expanded human umbilical cord blood-derived CD34+ cell therapy accelerates cutaneous wound closure in NOD/SCID mice. Journal of Cellular and Molecular Medicine. 2014; 18:685-97. doi: 10.1111/jcmm.12217. Epub 2014 Jan 23. (IF: 5.295)
Kanji S, Das M, Aggarwal R, Lu J, Joseph M, Basu S, Pompili VJ and Das H. Nanofiber-expanded human umbilical cord blood-derived CD34+ cell therapy accelerates murine cutaneous wound closure by attenuating pro-inflammatory factors and secreting IL-10. Stem Cell Research 2014; 12: 275-288. (IF: 1.587)
Knapik DM, Perera P, Nam J, Blazek AD, Rath B, Leblebicioglu B, Das H, Wu LC, Hewett TE, Agarwal SK Jr, Robling AG, Flanigan DC, Lee BS, Agarwal S. Mechanosignaling in Bone Health, Trauma and Inflammation. Forum Review Article in special issue, Mechanosignaling in bone health, trauma and inflammation. Antioxidants and Redox Signaling. 2014; 20: 970-985. doi: 10.1089/ars.2013.5467. (IF: 7.468)
Aggarwal R, Lu J, Kanji S, Das M, Joseph M, Lustberg, MB, Ray A, Pompili VJ, Shapiro CL and Das H. Human V纬2V未2 T Cells Limit Breast Cancer Growth by Modulating Cell Survival-, Apoptosis-Related Molecules, and Microenvironment in Tumors. International Journal of Cancer 2013; 133:2133-2144. (IF: 7.316)
Das H*, Wang Z, Niazi MKK, Aggarwal R, Lu J, Kanji S, Das M, Joseph M, Gurcan M* and Cristini V*. Impact of diffusion barriers to small cytotoxic molecules on the efficacy of immunotherapy in breast cancer (*corresponding author). PLoS One 2013; 8: e61398. doi:10.1371/journal.pone.0061398. (IF: 3.752)
Lu J, Kanji S, Aggarwal R, Das M, Joseph M, Wu LC, Mao HQ, Pompili VJ, Hadjiconstantinou M, and Das H. Hematopoietic stem cells improve dopaminergic neuron in the MPTP-mice. Frontiers in Bioscience 2013; 18: 970-981. DOI No:10.2741/4156. (IF: 3.115)
Lu J, Pompili VJ and Das H. Neovascularization and hematopoietic stem cells (Invited review, peer reviewed). Cell Biochemistry and Biophysics 2013: 67 (2): 235-45. DOI 10.1007/s12013-011-9298-x. (IF: 2.989)
Li Z, Guo X, Palmer AF, Das H, and Guan J. High Efficiency Matrix Modulus-Induced Cardiac Differentiation of Human Mesenchymal Stem Cells inside a Thermosensitive Hydrogel. Acta Biomaterialia 2012; 8(10): 3586-95. (IF: 10.633)
Aggarwal R, Lu J, Kanji S, Joseph M, Das M, Noble G, McMichael BK, Agarwal S, Hart RT, Sun Z, Lee BS, Jackson R, Mao HQ, Pompili VJ and Das H. Human umbilical cord blood-derived CD34+ stem cells reverse osteoporosis in NOD/SCID mice by altering osteoblastic and osteoclastic activities. PLoS One 2012; 7(6): e39365. (IF: 3.752)
Das M, Lu J, Joseph M, Aggarwal R, Kanji S, McMichael B, Lee BS, Agarwal S, Ray-Chaudhury A, Iwenofu, OH, Kuppusamy P, Pompili VJ, Jain MK and Das H. Kr眉ppel-like factor 2 (KLF2) regulates monocyte differentiation and functions in mBSA and IL-1尾-induced arthritis. Current Molecular Medicine 2012; 12 (2): 113-125. (IF: 2.616)
Aggarwal R, Lu J, Pompili VJ and Das H. Hematopoietic Stem Cells: Transcriptional Regulation, Ex vivo Expansion and Clinical Application (Invited review, peer reviewed). Current Molecular Medicine 2012; 12 (1): 34-49. (IF: 2.616)
Lu J, Aggarwal R, Kanji S, Das M, Joseph M, Pompili VJ and Das H. Human Ovarian Tumor Cells Escape 纬未 T Cell Recognition Partly by Down Regulating Surface Expression of MICA and Limiting Cell Cycle Related Molecules. PLoS One 2011; 6(9): e23348. doi:10.1371/journal.pone.0023348. (IF: 3.752)
Kanji S, Pompili VJ and Das H. Plasticity and maintenance of hematopoietic stem cells during development (Invited review, peer reviewed). Recent Patents on Biotechnology 2011; 5:40-53.
Kanji S, Pompili VJ and Das H. Recent advances in hematopoietic stem cell-mediated regeneration (Invited review, peer reviewed). Recent Patents on Regenerative Medicine 2011; 1: 195-206.
Adler DS, Lazarus H, Nair R, Goldberg JL, Greco NJ, Lassar T, Laughlin, MJ, Das H* and Pompili VJ. Safety and efficacy of bone marrow-derived autologous CD133+ stem cell therapy. Frontiers in Bioscience 2011; 3: 506-514. (*Corresponding author). (IF: 3.115)
Lu J, Aggarwal R, Pompili VJ and Das H. A novel technology for hematopoietic stem cell expansion using combination of nanofiber and growth factors. (Invited review, peer reviewed). Recent Patents on Nanotechnology 2010; 4: 125-134. (IF: 2.321)
Aggarwal R, Pompili VJ and Das H. Genetic modification of ex-vivo expanded stem cells for clinical application. (Invited review, peer reviewed). Frontiers in Bioscience 2010; 15: 854-871. (IF: 3.115)
Lu J, Pompili VJ and Das H. Hematopoietic stem cells: ex-vivo expansion and therapeutic potential for myocardial ischemia (Invited review, peer reviewed). Stem Cells and Cloning: Advances and Applications 2010; 3: 57-68.
Das H*, George JC, Joseph M, Das M, Abdulhameed N, Blitz, A, Sakthivel R, Mao HQ, Hoit BD, Kuppusamy P and Pompili VJ. Stem cell therapy with overexpressed VEGF and PDGF genes improves cardiac function in a rat infarct model. PLoS ONE 2009; 4 (10): e7325. Doi:10. (*Corresponding author). (IF: 3.752)
Das H*, Abdulhameed N, Joseph M, Sakthivel R, Mao HQ and Pompili VJ*. Ex-vivo nanofiber expansion and genetic modification of human cord blood-derived progenitor/stem cells enhances vasculogenesis. Cell Transplantation 2009; 18: 305-318. (*Corresponding author). (IF: 4.139)
George JC, Goldberg J, Joseph M, Abdulhameed N, Crist J, Das H* and Pompili VJ*. Transvenous Intramyocardial Cellular Delivery Increases Retention in Comparison to Intracoronary Delivery in a Porcine Model of Acute Myocardial Infarction. Journal of Interventional Cardiology 2008; 21: 424-431 (*Corresponding author). (IF: 1.776)
Fisch S, Gray S, Heymans S, Halder S, Wang B, Pfister O, Cui L, Kumar A, Lin Z, Sen-Banerjee S, Das H, Petersen CA, Mende U, Burleigh BA, Zhu Y, Pinto Y, Liao R and Jain MK. Kr眉ppel-like factor 15 is a novel regulator of cardiomyocyte hypertrophy. Proceedings of the National Academy of Sciences, 2007; 104: 7074-7079. (IF: 12.779)
Das H, Kumar A, Lin Z, Patino WD, Hwang PM, Feinberg MW, Majumder PK and Jain MK. Kr眉ppel-like factor 2 (KLF2) regulates proinflammatory activation of monocytes. Proceedings of the National Academy of Sciences, 2006; 103: 6653-6658. (IF: 12.779)
Sen-banerjee S, Mir S, Lin Z, Hamik A, Atkins GB, Das H, Banerjee P, Kumar A and Jain MK. Kr眉ppel-like factor 2 as a novel mediator of statin effects in endothelial cells. Circulation 2005; 112:720-726. (IF: 39.918)
Bukowski JF, Dascher CC, Das H. Alternative bisphosphonate targets and mechanisms of action (review). Biochemical and Biophysical Research Communications 2005; 328: 746-750. (IF: 3.322)
Das H, Sugita M and Brenner MB. Mechanisms of Vd1 gd T cell activation by microbial components. Journal of Immunology 2004, 172: 6578-6586. (IF: 5.446)
Kamath AB, Wang L, Das H, Li L, Reinhold VN and Bukowski JF. Antigens in tea-beverage prime human Vg2Vd2 T cells in vitro and in vivo for memory and nonmemory antibacterial cytokine responses. Proceedings of the National Academy of Sciences 2003, 100: 6009-6014. (IF: 12.779)
Narazaki H, Watari E, Shimizu M, Owaki A, Das H, Fukunaga Y, Takahashi H and Sugita M. Perforin-dependent killing of tumor cells by Vg1Vd1-bearing T cells. Immunology Letters 2002, 86: 113-119. (IF: 4.230)
Leslie DS, Vincent MS, Spada FM, Das H, Sugita M, Morita CT and Brenner MB. CD1-mediated gd T cell maturation of dendritic cells. Journal of Experimental Medicine 2002, 196:1575-1584. (IF: 17.579)
Wang L, Das H, Kamath A, Li L and Bukowski JF. Human Vg2Vd2 T cells augment MIF secretion, and counteract the inhibitory effect of glucocorticoids on IL-1b and TNF-a production. Journal of Immunology 2002, 168:4889-4896. (IF: 5.446)
Wang L, Das H, Kamath A and Bukowski JF. Human Vg2Vd2 T cells produce IFN-g and TNF-a with an on/off/on cycling pattern in response to live bacterial product. Journal of Immunology 2001, 167:6195-6201. (IF: 5.446)
Wang L, Kamath A, Das H, Li L and Bukowski JF. Antibacterial effect of human Vg2Vd2 T cells in vivo. Journal of Clinical Investigation 2001, 108:1349-1357. (IF: 19.486)
Das H, Wang L, Kamath A and Bukowski JF. Vg2Vd2 T cell receptor-mediated recognition of aminobisphosphonates. Blood 2001, 98:1616-1618. (IF: 25.476)
Das H, Groh V, Kuijl C, Sugita M, Morita C T, Spies T and Bukowski JF. MICA engagement by human Vg2Vd2 T cells enhances their antigen-dependent effector function. Immunity 2001, 15: 83-93. (IF: 43.474)
Das H, Imoto S, Murayama T, Kajimoto K, Sugimoto T, Isobe T, Nakagawa T, Nishimura R and Koizumi T. Kinetic analysis of cytokine gene expression during development of human graft versus host disease after donor lymphocyte transfusion. Bone Marrow Transplantation 2001, 27: 373-380. (IF: 5.176)
Taniguchi R, Koizumi T, Das H, Chakraborty S, Sugimoto T, Hasegawa K, Kono M and Nishimura R. Trophoblastic cells expressing human chorionic gonadotropin genes in peripheral blood of patients with trophoblastic disease. Life Sciences 2000, 66: 1593-1601. (IF: 6.780)
Das H, Koizumi T, Sugimoto T, Yamaguchi S, Hasegawa K, Tenjin Y and Nishimura R. Induction of apoptosis and manganese superoxide dismutase gene by photodynamic therapy in cervical carcinoma cell lines. International Journal of Clinical Oncology 2000, 5: 97-103. (IF: 3.850)
Imoto S, Oomoto Y, Murata K, Das H, Murayama T, Kajimoto K, Sugimoto T, Gomyo H, Nakagawa T, Nishimura R and Koizumi T. Kinetics of serum cytokines after allogeneic bone marrow transplantation: interleukin-5 as a potential marker of acute graft-versus-host disease. International Journal of Hematology 2000, 72: 92-97. (IF: 2.329)
Sugimoto T, Das H, Imoto S, Murayama T, Gomyo H, Chakraborty S, Taniguchi R, Isobe T, Nakagawa T, Nishimura R and Koizumi T. Quantitation of minimal residual disease in t(8;21)-positive acute myelogenous leukemia patients using real-time quantitative RT-PCR. American Journal of Hematology 2000, 64: 101-106. (IF: 13.265)
Das H, Koizumi T, Sugimoto T, Chakraborty S, Ichimura T, Hasegawa K and Nishimura R. Quantitation of Fas and Fas ligand gene expression in human ovarian, cervical and endometrial carcinomas using real-time quantitative RT-PCR. British Journal of Cancer 2000, 82: 1682-1688. (IF: 9.089)
Murayama T, Koizumi T, Das H, Kobayashi Y, Kajimoto K, Sugimoto T, Imoto S, Nishimura R and Nakagawa T. Soluble fas ligand in natural killer cell lymphoma. American Journal of Hematology 1999, 62: 253-255. (IF: 13.265)
Das H, Imoto S, Murayama T, Kajimoto K, Sugimoto T, Isobe T, Nakagawa T, Nishimura R and Koizumi T. Levels of soluble FasL and FasL gene expression during the development of graft-versus-host disease in DLT-treated patients. British Journal of Haematology 1999, 104: 795-800. (IF: 8.615)
Das H and Bhattacharyya AK. The effect of inhibitors; sodium fluoride and L (+) tartrate on purified acid phosphatase from normal and benign hyperplastic prostates. Journal of Basic and Applied Biomedicine 1995, 3(1): 19-22.
Das H and Bhattacharyya AK. Changes in biochemical markers of hyperplastic and malignant prostatic tissues. Archives of Andrology 1991, 26:185-188. (IF: 0.891)

Edited Books

Book entitled 鈥淢ethods in Molecular Biology: Wound Regeneration鈥�. Nature/Springer Protocols, editor Hiranmoy Das, PhD. Humana Press, New York, NY. 2020. ISBN 978-1-07-160844-9.
Book entitled 鈥淏one Regeneration: Concepts, Clinical Aspects and Future Directions. Nova Science Publishers Inc, editor Hiranmoy Das, PhD. Hauppauge, NY 11788 USA. 2018. ISBN: 978-1-53613-990-7.

Book Chapters

Sarkar J and Das H. The role of endothelin axis and reactive oxygen species in future therapies of pancreatic cancer. In Handbook of Oxidative Stress in Cancer: Therapeutic Aspect. Springer Nature. 2022.
Anderson S, Barthels D, and Das H. Kr眉ppel-like factor 2 and matrix metalloproteinases in the context of vasculature. In 鈥淢atrix Pathobiology and Angiogenesis鈥�. Springer Nature. 2022 -In press.
Barthels D, and Das H. Glial cells in neuroinflammation in various disease states. In 鈥淪tem cells: From Promise to Realism鈥�. Springer Nature. 2022 -In press.
Anderson S, Jha P, and Das H. Molecular Mechanisms of Osteoclasts and Osteoblasts in Bone Remodeling and Pathogenesis. In Bone Regeneration: Concepts, Clinical Aspects and Future Directions. Nova Science Publishers Inc, editor Hiranmoy Das. Hauppauge, NY 11788 USA. Chapter 1, pages 1-24, 2018.
Rolph DN and Das H. Nanofiber-Expanded CD34+ Stem Cells for Osteoporosis Therapy. In Bone Regeneration: Concepts, Clinical Aspects and Future Directions. Nova Science Publishers Inc, editor Hiranmoy Das. Hauppauge, NY 11788 USA. Chapter 9, pages 281-324, 2018.
Bhowmik A*, Laha D*, and Das H. Current Advances in Breast Cancer Therapeutics: Implication of Gamma Delta T Cells. In Targeted Cancer Therapy. SM Group Publishers. Dover, DE, USA. Chapter 6, pages 1-18, 2017. (*Equal Contribution).
Rolph DN, and Das H. Kr眉ppel-like factor 2 (KLF2) in limiting inflammation in rheumatoid arthritis. In Rheumatoid Arthritis. Avid Science Publication. Berlin, Germany. Chapter 2, pages 1-43, 2017.
Greene CJ, Rolph DN, and Das H. Stem cells and their preclinical applications for degenerative diseases. In Recent Advances in Stem Cell Research. Avid Science Publication. Berlin, Germany. Chapter 2, pages 1-45, 2017.
Rolph DN, Greene CJ, and Das H. Nanofiber expanded CD34+ stem cells as an alternative for bone marrow transplantation. In Bone Marrow Transplantation. Avid Science Publication. Berlin, Germany. Pages 1-37. 2016.
Lu J, Pompili VJ and Das H. Genetic engineering of hematopoietic stem cells to enhance functionality. In Stem Cell Engineering Handbook. Xuejun Wen Ed. New York, NY, USA: CRC press. ISBN: 9781420090055, 2012.
Aggarwal R, Pompili VJ, and Das H. Hematopoietic Stem Cells and Bone Regeneration, in Stem Cells and Cancer Stem Cells: Therapeutic Applications in Disease and Injury. M.A. Hayat Ed. Vol 11, Springer Publishing Co, New York, NY. 16: 189-199. DOI 10.1007/978-94-007-7329-5_16, 2014.
Lu J, Pompili VJ and Das H. Vascular Stem Cells in Regulation of Angiogenesis in Advances in Biochemistry in Health and Disease: Biochemical Basis and Therapeutic Implications of Angiogenesis. JL Mehta and NS Dhalla Eds, Springer Publishing Co, New York, NY. 8: 123-138. DOI 10.1007/978-1-4614-5857-9_8, 2013.
Aggarwal R, Pompili VJ, and Das H. Hematopoietic Stem Cells in Atherosclerotic Development and Resolution in Chronic Inflammation: Molecular Pathophysiology, Nutritional and Therapeutic Interventions. Roy S, Bagchi D, and Roychaudhuri SP Eds. CRC Press, New York, NY; 6: 77-91, 2012. ISBN-13: 978-1439872116.
Kanji S, Pompili VJ and Das H. Ischemia, Reactive Radicals, Redox Signaling and Hematopoietic Stem Cells in Stem Cells and Human Diseases. R. Srivastava and S. Shankar (eds.), Springer Publishing Co, New York, NY; 9: 197-216. ISBN 978-94-007-2800-4. DOI 10.1007/978-94-007-2801-1_9, 2012.
Lu J, Pompili VJ and Das H. gd T Cells, Tea and Cancer. Nutrition, Diet and Cancer. S. Shankar and R. Srivastava Eds, Springer Publishing Co, New York, NY; 8: 169-184. DOI 10.1007/978-94-007-2923-0_8, 2012.
Lu J, Aggarwal R, Pompili VJ and Das H. Ex-vivo expanded hematopoietic stem cells for ischemia. Stem Cells and Cancer Stem Cells: Therapeutic Applications in Disease and Injury. M.A. Hayat Ed, Springer Publishing Co, New York, NY; 23: 219-229. ISBN: 978-94-007-2015-2. DOI 10.1007/978-94-007-2016-9_23, 2012.
Aggarwal R, Lu J, Pompili VJ and Das H. Development of ischemic diseases and stem cell therapy. Natural Products and their Active Compounds on Disease Prevention. M. Mohamed Essa, A. Manickavasagan, and E. Sukumar edited, Nova Science Publishers, New York, NY, USA; 4: 71-89. ISBN: 978-1-62100-153-9, 2012.
Nishimura R, Koizumi T, Das H, Takemori M and Hasegawa K. Enzyme immunoassay of urinary b-core fragment of human chorionic gonadotropin as a tumor marker for ovarian cancer. In Methods in Molecular Medicine, Ovarian Cancer: Methods and Protocols, Bartlett JMS Ed, Humana Press, Inc., Totowa, NJ. 39:135-141, 2000.

1. Das H and Rush SW. Corneal epithelial cells and their products for treating corneal diseases. Patent # US 2021/0189334 A1 Publication date 06-24-2021.

2. Das H. Stem cells for the treatment for conditions and diseases. Patent # WO 2019/241462 A1. Publication date 12-19-2019.

3. Wright SE and Das H. Nonreleased IL-12 for therapy of cancer. Patent # US 2019 / 0091310 A. Publication Date 3-28-2019.

4. Sakthivel R, Brown DJ, Mao HQ, Douay L, Pompili VJ, Mclntosh K, Das H, Zhao Y. 鈥淓rythrocytes differentiated in vitro from nanofiber expanded CD133+ cells鈥�. Patent # 8669106. Publication date: 03-11-2014.

5. Sakthivel R, Brown DJ, Mao HQ, Douay L, Pompili VJ, Mclntosh K, Das H, Zhao Y. 鈥淢ethods and systems for expanding AC133+ cells and directing differentiation鈥�. Patent # 20090285892. Publication date: 11-19-2009.

updated: 10/11/2022

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